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KMID : 0363620110320010141
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Journal of Korean Oriental Medicine
2011 Volume.32 No. 1 p.141 ~ p.150
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Evaluation of Antioxidant Activity of Solvent Fractions of Angelica gigas Root Using TOSC Assay
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Lee Sook-Young
Seo Young-Bae
Woo Won-Hong
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Abstract
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Objectives: The aim of this study was to elucidate the antioxidant effect of solvent fractions of Angelica gigas root.
Methods: The ethanol extract of Angelica gigas root was suspended in water and then partitioned with dichloromethane (MC Fr.), ethyl acetate (EA Fr.) and butanol (BuOH Fr.), sequentially. The antioxidant activities of solvent fractions of Angelica gigas root were evaluated for radical scavenging activity against stable free radicals (2,2-diphenyl-1-picrylhydrazyl) DPPH, hydrogen peroxide and superoxide anion radicals. In addition the antioxidant activities of solvent fractions of Angelica gigas root against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay.
Results: Among the solvent fractions of MC Fr., EA Fr., and BuOH Fr., BuOH Fr. was found to have stronger antioxidant activity with values of 59.72, 14.36, 30.96 and on the DPPH radical, nitrite, superoxide anion and hydrogen peroxide, respectively, than BHA used as a positive control. Moreover, specific TOSC values(564.8, 276.4 and 405.5 TOSC/mM) of BuOH fr. against peroxyl radicals, hydroxyl radical and peroxynitrite were 4 times higher than GSH (136.5, 67.4 102.6 TOSC/mM) used as a positive control.
Conclusions: These results suggest that the BuOH fr. of Angelica gigas root has a high antioxidant activity and can be useful to develop functional food against oxidative stress conditions.
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KEYWORD
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Angelica gigas root, BuOH Fr., antioxidant activity, specific TOSC
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